DMSO is widely used to solubilize drugs of therapeutic applications and studies indicated that 10% v/v concentration did not modify culture viability when used to treat human peripheral blood mononuclear cells (PBMC). Copyright Â© 2014 John Wiley & Sons, Ltd.ĭimethyl Sulfoxide ( DMSO) Decreases Cell Proliferation and TNF-Î±, IFN-Î³, and IL-2 Cytokines Production in Cultures of Peripheral Blood Lymphocytes.ĭe Abreu Costa, Lucas Henrique Fernandes Ottoni, Marcelo Dos Santos, Michaelle Geralda Meireles, Agnes Batista Gomes de Almeida, ValÃ©ria de FÃ¡tima Pereira, Wagner Alves de Avelar-Freitas, BethÃ¢nia EustÃ¡quio Alvim Brito-Melo, Gustavoĭimethylsulfoxide ( DMSO) is an amphipathic molecule composed of a polar domain characterized by the sulfinyl and two nonpolar methyl groups, for this reason it is able to solubilize polar and nonpolar substances and transpose hydrophobic barriers. In conclusion, we reassessed DMSO concentration limits for different experimental endpoints in the planarian S. In the second part of our study, we propose a statistical approach to account for solvent-toxicant interactions and discuss full-scale solvent toxicity studies. As small sensitivity differences exist between biological levels and developmental stages, we advise the use of this solvent in concentrations below 500â€‰Âµlâ€‰l(-1) in this organism. After short exposure times, 500â€‰Âµlâ€‰l(-1) DMSO only affected motility, whereas the most significant changes on different parameters were observed at a concentration of 1000â€‰Âµlâ€‰l(-1) DMSO. behaviour, stem cell proliferation and gene expression profiles. In a long-term exposure set-up, a concentration of 500â€‰Âµlâ€‰l(-1) DMSO interfered with processes on different biological levels, e.g. We measured different biological levels in both fully developed and developing animals. In this study, the effects of both short- (1â€‰day) and long-term (2â€‰weeks) exposures to low DMSO concentrations (up to 1000â€‰Âµlâ€‰l(-1) ) were studied in the planarian Schmidtea mediterranea.

Hence, to avoid solvent-toxicant interference, we use multiple chronic test endpoints for additional interpretation of DMSO concentrations and propose a statistical model to assess possible synergistic, antagonistic or additive effects of test compounds and their solvents. DMSO concentration guidelines are available, but are mostly based on acute exposures involving one specific toxicity endpoint. However, using these solvents, the interpretation of test compound-induced responses can be biased. To investigate hydrophobic test compounds in toxicological studies, solvents like dimethylsulfoxide ( DMSO) are inevitable. Stevens, An-Sofie Pirotte, Nicky Plusquin, Michelle Willems, Maxime Neyens, Thomas Artois, Tom Smeets, Karen Toxicity profiles and solvent-toxicant interference in the planarian Schmidtea mediterranea after dimethylsulfoxide ( DMSO) exposure. Raising awareness of this significant issue in the cancer biology community is critical, and we make recommendations on appropriate solvation of platinum drugs for research. These results render a substantial portion of the literature on cisplatin uninterpretable. We examined the effect of DMSO on platinum complexes, including cisplatin, carboplatin and oxaliplatin, finding that DMSO reacted with the complexes, inhibited their cytotoxicity and their ability to initiate cell death. However, dissolving cisplatin in DMSO for laboratory-based studies results in ligand displacement and changes the structure of the complex. In this study, we examined the literature and found a significant number of studies (11 - 34%) in prominent cancer journals utilizing cisplatin dissolved in dimethylsulfoxide ( DMSO). The platinum drugs cisplatin, carboplatin and oxaliplatin are highly utilized in the clinic and as a consequence are extensively studied in the laboratory setting. Say No to DMSO: Dimethylsulfoxide Inactivates Cisplatin, Carboplatin and Other Platinum Complexes

Dmso molar mass periodic table print